Updated: Nov-12-2024
MotifPeeker-reports
is a public archive for interactive HTML reports generated by MotifPeeker
,
along with the code used to produce them.
Reports
CTCF ChIP-seq - TIP-seq
Dataset Details
Compares three public ChIP-seq datasets against a TIP-seq dataset for CTCF in HCT116 cells. Details of the datasets used are as follows:
-
ChIPSeq1
ENCODE Accession: ENCFF172XLC
-
ChIPSeq2
ENCODE Accession: ENCFF411OOS
-
ChIPSeq3
ENCODE Accession: ENCFF091ODJ -
TIPSeq
Alignment file produced by running thenf-core/cutandrun
pipeline on the raw sequencing data sourced from NIH Sequence Read Archives ID: SRR16963166.
Peak calling was performed using MACS3
using the following commands:
## For ChIP-Seq datasets
MACSr::callpeak(
tfile = ...,
cfile = NULL, # No control file
nomodel = FALSE,
qvalue = 0.01,
format = "BAM",
name = "chipseq",
outdir = ...
)
## For TIP-Seq dataset
MACSr::callpeak(
tfile = tipseq_alignment_data,
nomodel = TRUE,
qvalue = 0.01,
shift = -75, # 5' shift
extsize = 150,
keepduplicates = "all",
format = "BAM",
name = "tipseq",
outdir = ...
)
Motif files used:
Built-in data
Dataset Details
Report generated from built-in data, as described in theMotifPeeker
vignette.
Session Info
utils::sessionInfo()
## R version 4.4.1 (2024-06-14)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 22.04.4 LTS
##
## Matrix products: default
## BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.20.so; LAPACK version 3.10.0
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## time zone: UTC
## tzcode source: system (glibc)
##
## attached base packages:
## [1] stats graphics grDevices utils datasets methods base
##
## loaded via a namespace (and not attached):
## [1] digest_0.6.37 desc_1.4.3 R6_2.5.1 fastmap_1.2.0
## [5] xfun_0.49 cachem_1.1.0 knitr_1.49 htmltools_0.5.8.1
## [9] rmarkdown_2.29 lifecycle_1.0.4 cli_3.6.3 sass_0.4.9
## [13] pkgdown_2.1.1 textshaping_0.4.0 jquerylib_0.1.4 systemfonts_1.1.0
## [17] compiler_4.4.1 tools_4.4.1 ragg_1.3.2 bslib_0.8.0
## [21] evaluate_1.0.1 yaml_2.3.10 jsonlite_1.8.9 rlang_1.1.4
## [25] fs_1.6.5 htmlwidgets_1.6.4