Installation

MSTExplorer is now available via ghcr.io as a containerised environment with Rstudio and all necessary dependencies pre-installed.

Method 1: via Docker

First, install Docker if you have not already.

Create an image of the Docker container in command line:

docker pull ghcr.io/neurogenomics/MSTExplorer

Once the image has been created, you can launch it with:

docker run \
  -d \
  -e ROOT=true \
  -e PASSWORD="<your_password>" \
  -v ~/Desktop:/Desktop \
  -v /Volumes:/Volumes \
  -p 8900:8787 \
  ghcr.io/neurogenomics/MSTExplorer

NOTES

  • Make sure to replace <your_password> above with whatever you want your password to be.
  • Change the paths supplied to the -v flags for your particular use case.
  • The -d ensures the container will run in “detached” mode, which means it will persist even after you’ve closed your command line session.
  • The username will be “rstudio” by default.
  • Optionally, you can also install the Docker Desktop to easily manage your containers.

Method 2: via Singularity

If you are using a system that does not allow Docker (as is the case for many institutional computing clusters), you can instead install Docker images via Singularity.

singularity pull docker://ghcr.io/neurogenomics/MSTExplorer

For troubleshooting, see the Singularity documentation.

Usage

Finally, launch the containerised Rstudio by entering the following URL in any web browser: http://localhost:8900/

Login using the credentials set during the Installation steps.

Session Info

utils::sessionInfo()
## R Under development (unstable) (2025-11-30 r89082)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.3 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] MSTExplorer_1.0.10
## 
## loaded via a namespace (and not attached):
##   [1] later_1.4.4                 bitops_1.0-9               
##   [3] ggplotify_0.1.3             GeneOverlap_1.47.0         
##   [5] filelock_1.0.3              tibble_3.3.0               
##   [7] lifecycle_1.0.4             httr2_1.2.1                
##   [9] KGExplorer_0.99.10          rstatix_0.7.3              
##  [11] HPOExplorer_1.0.6           doParallel_1.0.17          
##  [13] lattice_0.22-7              pals_1.10                  
##  [15] backports_1.5.0             magrittr_2.0.4             
##  [17] limma_3.67.0                plotly_4.11.0              
##  [19] sass_0.4.10                 rmarkdown_2.30             
##  [21] jquerylib_0.1.4             yaml_2.3.11                
##  [23] httpuv_1.6.16               otel_0.2.0                 
##  [25] HGNChelper_0.8.15           mapproj_1.2.12             
##  [27] DBI_1.2.3                   RColorBrewer_1.1-3         
##  [29] maps_3.4.3                  abind_1.4-8                
##  [31] GenomicRanges_1.63.0        rvest_1.0.5                
##  [33] purrr_1.2.0                 BiocGenerics_0.57.0        
##  [35] yulab.utils_0.2.2           rappdirs_0.3.3             
##  [37] gdtools_0.4.4               circlize_0.4.16            
##  [39] IRanges_2.45.0              S4Vectors_0.49.0           
##  [41] rols_2.99.5                 tidytree_0.4.6             
##  [43] piggyback_0.1.5             pkgdown_2.2.0              
##  [45] DelayedArray_0.37.0         codetools_0.2-20           
##  [47] xml2_1.5.1                  tidyselect_1.2.1           
##  [49] shape_1.4.6.1               aplot_0.2.9                
##  [51] farver_2.1.2                matrixStats_1.5.0          
##  [53] stats4_4.6.0                BiocFileCache_3.1.0        
##  [55] Seqinfo_1.1.0               jsonlite_2.0.0             
##  [57] GetoptLong_1.1.0            tidygraph_1.3.1            
##  [59] Formula_1.2-5               iterators_1.0.14           
##  [61] systemfonts_1.3.1           foreach_1.5.2              
##  [63] tools_4.6.0                 treeio_1.35.0              
##  [65] ragg_1.5.0                  Rcpp_1.1.0                 
##  [67] glue_1.8.0                  SparseArray_1.11.6         
##  [69] xfun_0.54                   MatrixGenerics_1.23.0      
##  [71] RNOmni_1.0.1.2              dplyr_1.1.4                
##  [73] BiocManager_1.30.27         fastmap_1.2.0              
##  [75] caTools_1.18.3              digest_0.6.39              
##  [77] R6_2.6.1                    mime_0.13                  
##  [79] gridGraphics_0.5-1          textshaping_1.0.4          
##  [81] colorspace_2.1-2            gtools_3.9.5               
##  [83] dichromat_2.0-0.1           RSQLite_2.4.5              
##  [85] tidyr_1.3.1                 generics_0.1.4             
##  [87] fontLiberation_0.1.0        data.table_1.17.8          
##  [89] S4Arrays_1.11.1             httr_1.4.7                 
##  [91] htmlwidgets_1.6.4           scatterplot3d_0.3-44       
##  [93] pkgconfig_2.0.3             gtable_0.3.6               
##  [95] blob_1.2.4                  ComplexHeatmap_2.27.0      
##  [97] S7_0.2.1                    SingleCellExperiment_1.33.0
##  [99] XVector_0.51.0              htmltools_0.5.8.1          
## [101] fontBitstreamVera_0.1.1     carData_3.0-5              
## [103] clue_0.3-66                 scales_1.4.0               
## [105] Biobase_2.71.0              png_0.1-8                  
## [107] ggfun_0.2.0                 knitr_1.50                 
## [109] reshape2_1.4.5              rjson_0.2.23               
## [111] nlme_3.1-168                curl_7.0.0                 
## [113] cachem_1.1.0                GlobalOptions_0.1.3        
## [115] Polychrome_1.5.4            stringr_1.6.0              
## [117] BiocVersion_3.23.1          KernSmooth_2.23-26         
## [119] parallel_4.6.0              AnnotationDbi_1.73.0       
## [121] desc_1.4.3                  pillar_1.11.1              
## [123] grid_4.6.0                  vctrs_0.6.5                
## [125] gplots_3.3.0                promises_1.5.0             
## [127] ggpubr_0.6.2                car_3.1-3                  
## [129] dbplyr_2.5.1                xtable_1.8-4               
## [131] cluster_2.1.8.1             evaluate_1.0.5             
## [133] orthogene_1.17.0            cli_3.6.5                  
## [135] compiler_4.6.0              rlang_1.1.6                
## [137] crayon_1.5.3                grr_0.9.5                  
## [139] simona_1.9.0                ggsignif_0.6.4             
## [141] gprofiler2_0.2.4            plyr_1.8.9                 
## [143] EWCE_1.19.0                 fs_1.6.6                   
## [145] ggiraph_0.9.2               stringi_1.8.7              
## [147] viridisLite_0.4.2           ewceData_1.19.0            
## [149] BiocParallel_1.45.0         babelgene_22.9             
## [151] Biostrings_2.79.2           lazyeval_0.2.2             
## [153] homologene_1.4.68.19.3.27   fontquiver_0.2.1           
## [155] Matrix_1.7-4                ExperimentHub_3.1.0        
## [157] patchwork_1.3.2             bit64_4.6.0-1              
## [159] ggplot2_4.0.1               statmod_1.5.1              
## [161] KEGGREST_1.51.1             shiny_1.11.1               
## [163] SummarizedExperiment_1.41.0 AnnotationHub_4.1.0        
## [165] igraph_2.2.1                broom_1.0.10               
## [167] memoise_2.0.1               bslib_0.9.0                
## [169] ggtree_4.1.1                fastmatch_1.1-6            
## [171] bit_4.6.0                   splitstackshape_1.4.8      
## [173] ape_5.8-1