Installation

MultiEWCE is now available via ghcr.io as a containerised environment with Rstudio and all necessary dependencies pre-installed.

Method 1: via Docker

First, install Docker if you have not already.

Create an image of the Docker container in command line:

docker pull ghcr.io/neurogenomics/MultiEWCE

Once the image has been created, you can launch it with:

docker run \
  -d \
  -e ROOT=true \
  -e PASSWORD="<your_password>" \
  -v ~/Desktop:/Desktop \
  -v /Volumes:/Volumes \
  -p 8900:8787 \
  ghcr.io/neurogenomics/MultiEWCE

NOTES

  • Make sure to replace <your_password> above with whatever you want your password to be.
  • Change the paths supplied to the -v flags for your particular use case.
  • The -d ensures the container will run in “detached” mode, which means it will persist even after you’ve closed your command line session.
  • The username will be “rstudio” by default.
  • Optionally, you can also install the Docker Desktop to easily manage your containers.

Method 2: via Singularity

If you are using a system that does not allow Docker (as is the case for many institutional computing clusters), you can instead install Docker images via Singularity.

singularity pull docker://ghcr.io/neurogenomics/MultiEWCE

For troubleshooting, see the Singularity documentation.

Usage

Finally, launch the containerised Rstudio by entering the following URL in any web browser: http://localhost:8900/

Login using the credentials set during the Installation steps.

Session Info

utils::sessionInfo()
## R Under development (unstable) (2023-11-08 r85496)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 22.04.3 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.20.so;  LAPACK version 3.10.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] MultiEWCE_0.1.9  BiocStyle_2.31.0
## 
## loaded via a namespace (and not attached):
##   [1] later_1.3.1                   bitops_1.0-7                 
##   [3] ggplotify_0.1.2               GeneOverlap_1.39.0           
##   [5] filelock_1.0.2                tibble_3.2.1                 
##   [7] ontologyPlot_1.6              ggnetwork_0.5.12             
##   [9] graph_1.81.0                  lifecycle_1.0.4              
##  [11] rstatix_0.7.2                 HPOExplorer_0.99.12          
##  [13] rprojroot_2.0.4               lattice_0.22-5               
##  [15] backports_1.4.1               magrittr_2.0.3               
##  [17] limma_3.59.1                  plotly_4.10.3                
##  [19] sass_0.4.7                    rmarkdown_2.25               
##  [21] jquerylib_0.1.4               yaml_2.3.7                   
##  [23] httpuv_1.6.12                 HGNChelper_0.8.1             
##  [25] DBI_1.1.3                     RColorBrewer_1.1-3           
##  [27] abind_1.4-5                   zlibbioc_1.49.0              
##  [29] GenomicRanges_1.55.1          purrr_1.0.2                  
##  [31] BiocGenerics_0.49.1           RCurl_1.98-1.13              
##  [33] yulab.utils_0.1.0             rappdirs_0.3.3               
##  [35] GenomeInfoDbData_1.2.11       IRanges_2.37.0               
##  [37] S4Vectors_0.41.1              tidytree_0.4.5               
##  [39] piggyback_0.1.5               pkgdown_2.0.7                
##  [41] DelayedArray_0.29.0           tidyselect_1.2.0             
##  [43] aplot_0.2.2                   matrixStats_1.1.0            
##  [45] stats4_4.4.0                  BiocFileCache_2.11.1         
##  [47] jsonlite_1.8.7                ellipsis_0.3.2               
##  [49] systemfonts_1.0.5             paintmap_1.0                 
##  [51] tools_4.4.0                   treeio_1.27.0                
##  [53] ragg_1.2.6                    Rcpp_1.0.11                  
##  [55] glue_1.6.2                    SparseArray_1.3.1            
##  [57] xfun_0.41                     MatrixGenerics_1.15.0        
##  [59] GenomeInfoDb_1.39.1           RNOmni_1.0.1.2               
##  [61] dplyr_1.1.3                   BiocManager_1.30.22          
##  [63] fastmap_1.1.1                 fansi_1.0.5                  
##  [65] caTools_1.18.2                digest_0.6.33                
##  [67] R6_2.5.1                      mime_0.12                    
##  [69] gridGraphics_0.5-1            textshaping_0.3.7            
##  [71] colorspace_2.1-0              gtools_3.9.4                 
##  [73] RSQLite_2.3.3                 utf8_1.2.4                   
##  [75] tidyr_1.3.0                   generics_0.1.3               
##  [77] data.table_1.14.8             httr_1.4.7                   
##  [79] htmlwidgets_1.6.2             S4Arrays_1.3.0               
##  [81] ontologyIndex_2.11            pkgconfig_2.0.3              
##  [83] gtable_0.3.4                  blob_1.2.4                   
##  [85] SingleCellExperiment_1.25.0   XVector_0.43.0               
##  [87] htmltools_0.5.7               carData_3.0-5                
##  [89] bookdown_0.36                 scales_1.2.1                 
##  [91] Biobase_2.63.0                png_0.1-8                    
##  [93] ggfun_0.1.3                   knitr_1.45                   
##  [95] reshape2_1.4.4                coda_0.19-4                  
##  [97] statnet.common_4.9.0          nlme_3.1-163                 
##  [99] curl_5.1.0                    cachem_1.0.8                 
## [101] stringr_1.5.0                 BiocVersion_3.19.1           
## [103] KernSmooth_2.23-22            parallel_4.4.0               
## [105] AnnotationDbi_1.65.2          desc_1.4.2                   
## [107] pillar_1.9.0                  grid_4.4.0                   
## [109] vctrs_0.6.4                   gplots_3.1.3                 
## [111] promises_1.2.1                ggpubr_0.6.0                 
## [113] car_3.1-2                     dbplyr_2.4.0                 
## [115] xtable_1.8-4                  Rgraphviz_2.47.0             
## [117] evaluate_0.23                 orthogene_1.9.0              
## [119] cli_3.6.1                     compiler_4.4.0               
## [121] rlang_1.1.2                   crayon_1.5.2                 
## [123] grr_0.9.5                     ggsignif_0.6.4               
## [125] gprofiler2_0.2.2              EWCE_1.11.2                  
## [127] plyr_1.8.9                    fs_1.6.3                     
## [129] stringi_1.8.1                 viridisLite_0.4.2            
## [131] ewceData_1.11.0               network_1.18.1               
## [133] babelgene_22.9                munsell_0.5.0                
## [135] Biostrings_2.71.1             lazyeval_0.2.2               
## [137] homologene_1.4.68.19.3.27     Matrix_1.6-2                 
## [139] ExperimentHub_2.11.0          patchwork_1.1.3              
## [141] bit64_4.0.5                   ggplot2_3.4.4                
## [143] KEGGREST_1.43.0               statmod_1.5.0                
## [145] shiny_1.7.5.1                 SummarizedExperiment_1.33.0  
## [147] interactiveDisplayBase_1.41.0 AnnotationHub_3.11.0         
## [149] broom_1.0.5                   memoise_2.0.1                
## [151] bslib_0.5.1                   ggtree_3.11.0                
## [153] bit_4.0.5                     ape_5.7-1